Sequencing - Primer Walking Services

In our Primer walk service, we conduct initial primer extension reactions from universal or customer specified primers for plasmid or PCR products greater than 1 kb in length. Unique primers corresponding to the terminal sequence obtained from the initial reaction will be synthesized by Eton and additional reactions will be performed as necessary to completely sequence the region of interest. Single stranded walks or double stranded walks are available.

Publication Quality Sequencing Service is also available for plasmid and PCR Products. We will perform primer walk sequencing reactions as described above by using both DNA strands simultaneously when known initial primer sites exist on both terminal ends of the region of interest. Both strands of the region of interest are sequenced independently. The accuracy of Publication Quality Sequencing data is guaranteed by virtue of complete complementary sequences between the two strands.

Benefits of Primer Walking

Sample Submission

Turnaround Time

Varies by plasmid size. Our service yields approximately 1.5kb/week. We can provide the sequence data as it is generated.


$25 per walk after initial sequencing reaction cost. Cost covers the following services:

For price inquiries, please email

Placing Your Primer Walking Order:

***Please note that we cannot guarantee completion of primer walking projects, since secondary structures and difficult regions of sequence can make it impossible to read through certain sequences. However, we always try our best and if we cannot complete the project, we will only charge for the sequences obtained.

For additional questions about these services email

For help placing your order please call 1-800-758-1630.

Copyright © 2003-2020

All Rights Reserved

Eton Bioscience, Inc.
5820 Oberlin Drive, Suite 108
San Diego, CA 92121
DNA Sequencing
Specialty DNA Sequencing
Direct Sequencing
Genomic Services
Fragment Analysis
Mammilian Cell Expression
Peptide Synthesis
Antibody Services
DNA Prep Service
Facebook LinkedIn Google Plus Twitter