DNA Sequencing - Primer

Universal Primer List
Primer Name Primer Sequence 5'-3'
T7 Promotor TAA TAC GAC TCA CTA TAG GG
T7 Terminator GCT AGT TAT TGC TCA GCG G
T3 ATT AAC CCT CAC TAA AGG GA
SP6 GAT TTA GGT GAC ACT ATA G
M13F(-21) TGT AAA ACG ACG GCC AGT
M13F(-40) GTT TTC CCA GTC ACG AC
M13R Reverse CAG GAA ACA GCT ATG AC
AOX1 Forward GAC TGG TTC CAA TTG ACA AGC
AOX1 Reverse GCA AAT GGC ATT CTG ACA TCC
pGEX Forward (GST 5, pGEX 5') GGG CTG GCA AGC CAC GTT TGG TG
pGEX Reverse (GST 3, pGEX 3') CCG GGA GCT GCA TGT GTC AGA GG
BGH Reverse TAG AAG GCA CAG TCG AGG
GFP (C' terminal, CFP, YFP or BFP) CAC TCT CGG CAT GGA CGA GC
GFP Reverse TGG TGC AGA TGA ACT TCA GG
GAG GTT CGA CCC CGC CTC GAT CC
GAG Reverse TGA CAC ACA TTC CAC AGG GTC
CYC1 Reverse GCG TGA ATG TAA GCG TGA C
pFastBacF GGA TTA TTC ATA CCG TCC CA
pFastBacR CAA ATG TGG TAT GGC TGA TT
pBAD Forward ATG CCA TAG CAT TTT TAT CC
pBAD Reverse GAT TTA ATC TGT ATC AGG
CMV-Forward CGC AAA TGG GCG GTA GGC GTG
LKO.1 5' (hU6 promoter) GAC TAT CAT ATG CTT ACC GT

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