Mammalian Cell Expression Service
Eton now offers custom gene cloning, expression and stable cell-line development service. Using your desired vectors and clonal selection methods, we can isolate and express the desired gene for you and establish clonal cell-lines with the highest levels of target gene expression.
This service includes
- Isolation of gene of interest;
- Generation of recombinant construct;
- Verification of recombinant construct by restriction mapping and sequence analysis;
- Preparation of plasmid construct DNA;
- Introduce plasmid DNA into mamalian cells;
- Selection of over-expressing cells;
- Small scale expression and purification study assessed by SDS-PAGE and Western blot;
- Estimation of protein yield;
- Developing of stable cell line and expression of gene of interest.