From standard research primers to complex modified RNA — Eton connects researchers with the synthesis capabilities their research demands, backed by a support team at every step.
Eton provides highly flexible custom DNA and RNA oligonucleotide synthesis and delivery solutions. We support a wide range of chemical modifications, purification methods, and custom packaging/aliquoting options, allowing you to tailor each order to your exact requirements.
Our services offer scalable production from µg to kg quantities, along with rigorous quality control and verification. This flexibility makes our solutions well suited for applications in diagnostics, genome editing, sequencing, and synthetic biology.
Services
What we offer
DNA
Custom DNA Oligos
Short primers, probes, and NGS adapters for research, diagnostics, and gene editing. Available from 5 to 200 bases with multiple purification grades and fast turnaround.
High-quality RNA with diverse lengths and multiple modifications, including siRNA, sgRNA, ASO and custom RNA for gene editing, functional studies and nucleic acid research.
Over 200 chemical modification options — fluorophores, quenchers, backbone chemistries, affinity tags, and spacers — to support qPCR, antisense research, and advanced probe design.
All modified oligos are verified by mass spectometry and HPLC detection before shipment. Yoou recieve a complete Certificate of Analysis with every order - not as an an optional upgrade.
02
200+ Chemical Modifications
From simple fluorescent labels to complex backbone chemistries — our modification catalog covers the full range of current research and therapeutic applications.
03
Consistent Quality
Eton minimizes batch-to-batch variation and cross-contamination through strict controls in facility design, production processes, and workflows, ensuring consistent quality across all orders.
04
Every Order Matters
Eton enables rapid response and fast turnaround for your orders. We prioritize flexibility and responsiveness, ensuring every order is handled with care and attention.
Modification Catalog
200+ modification options
Fluorophores
30+
FAM · CY3 · CY5 · Alexa Fluor series · ATTO series · HEX · ROX · TXR · TAMRA
Desalt-purified primers for standard PCR. HPLC or PAGE purification required for qPCR hydrolysis probes — quencher-free truncated species elevate background signal and affect Cq values.
NGS Library Preparation
Barcode cross-contamination below 0.05%, verified per lot before shipment. Supporting hybridization-based capture and amplicon-based enrichment designs across major platforms.
CRISPR & Genome Editing
Chemically synthesized crRNA, tracrRNA, and sgRNA components. No DNA carryover — reducing unintended genomic integration risk present in IVT-derived guide components.
IVD & Diagnostics
Custom probes with complete contamination control documentation. E. coli and human DNA contamination testing, NTC signal minimization, and full analytical records for every lot.
Antisense & Therapeutic RNA
Gapmer and fully-modified ASO designs with full phosphorothioate backbone and 2′-OMe, MOE, LNA, or cEt wing modifications. Research to preclinical scale.
Gene Synthesis & Assembly
Building block oligos for gene assembly by overlap-extension PCR. High coupling efficiency and PAGE purification to remove truncated species before assembly use.
Ready to order or need a custom quote?
Our support team is available to assist with any inquiry.
Custom DNA Oligonucleotides — from research primers to diagnostic-grade probes
From µg research primers to kg custom oligos, Eton delivers custom DNA oligonucleotides across the full length range (5–200 bases) — all verified by mass spectrometry before shipment.
Reliable oligos for any molecular biology workflow
DNA oligonucleotides are indispensable molecular tools — as PCR primers, hybridization probes, NGS adapters, or the building blocks of gene synthesis. Each application places distinct demands on purity, sequence fidelity, and modification chemistry.
Eton manages the complete ordering workflow — from sequence submission through purification selection, quality verification, and delivery. Our team handles technical consultation, order tracking, and COA documentation, so your lab receives exactly what you ordered, when you need it.
Service Details
Formats & specifications
Service tier
Length
Purification
Delivery scale
Turnaround
Standard
5–60 nt
DSL / OPC / PAGE / HPLC
10 nmol–1 µmol
24–48 hrs
Long oligo
61–200 nt
PAGE / HPLC / Dual PAGE & HPLC
10 nmol–1 µmol
Per inquiry
Modified oligo
5–120 nt
PAGE / HPLC / Dual PAGE & HPLC
10 nmol–1 µmol
Per inquiry
Large-scale
Custom
Custom
mg–kg
Per inquiry
Purification guidance: Desalt is sufficient for primers ≤50 nt used in standard PCR. For oligos >60 nt, PAGE or HPLC purification is recommended to remove truncated species and ensure consistent downstream performance.
Modified oligo specifications — contact our team for details on specific modification chemistries, coupling efficiencies, and compatible purification grades.
Probe configurations including dual-labeled probe, double-quenched probe, and molecular beacon formats. Contact us for specifications and available fluorophore/quencher pairings.
NGS adapter and primer specifications available per platform. Contact our team for current adapter designs compatible with your sequencing workflow.
Large-scale synthesis from mg to kilogram quantities. The same quality standard applies — no reformulation or revalidation required for scale-up. Request a quote for pricing and lead times.
Order Workflow
From sequence to delivery
01
Submit your sequence
Online or via email. Specify purification grade and delivery scale.
02
Purification review
Our team confirms the right purification options for your application.
03
Quality verification
MS: Deviation ≤±0.3%; HPLC: Main peak area ≥90%
04
Delivery
Lyophilized or in solution, with COA and optional MS chromatogram.
Key Advantages
Built for reliability
01
Quality-verified delivery on every order
Every order verified by mass spectrometry before shipment. Full-length product confirmation and a detailed COA provided as standard — not as an optional upgrade.
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Four purification options, matched to your application
DSL, OPC, PAGE, and HPLC — including dual PAGE+HPLC for demanding applications. Our team recommends the right option for your downstream use case.
03
Scalable from research to production
Same sequence, same quality standard from µg to kilogram quantities. Scale-up does not require reformulation or revalidation of your order.
04
Technical support and sequencing
Application-specific guidance on purification, modification selection, and primer design. Sanger and long-read sequencing available from our laboratories.
Ready to place an order?
Contact our support team for technical consultation or custom quotes.
Custom RNA Oligonucleotides — siRNA, sgRNA, ASO, and beyond
From chemically synthesized siRNA and sgRNA to complex ASOs and modified RNA — all orders verified by mass spectrometry before shipment, with full modification integrity documentation.
Chemically synthesized RNA — ready to use, rigorously verified
RNA oligonucleotides play central roles in gene silencing (siRNA, miRNA mimics), genome editing (sgRNA), and antisense therapeutics (ASOs). Each application has distinct requirements for length, chemical stability, modification profile, and purity — all of which are addressed through appropriate synthesis chemistry and purification.
All RNA oligonucleotides are managed through an ordering and technical support workflow. Our team confirms modification specifications, purification selection, and delivery requirements before synthesis begins — and every product is mass spectrometry-verified before shipment.
Service Details
RNA formats & specifications
Format
Length
Specification
Note
Purification
Turnaround
Standard siRNA duplex
20-25 bp
5 nmol/2 OD
Standard unmodified siRNA
HPLC
Inquire
Chemically modified siRNA
20-25 bp
5 nmol/2 OD
Including fluorescent labeling and other chemical modifications
HPLC
Inquire
siRNA Sets
20-25 bp
5 nmol/2 OD
3 or 4 siRNAs+PC+NC
HPLC
Inquire
siRNA Control
20-25 bp
2.5 nmol/1 OD
Negative control sRNA, positive control siRNA, transfection control sRNA, and more available.
HPLC
Inquire
Process-grade
20-25 bp
≥20 nmol/8 OD
Large-scale synthesis
HPLC
Inquire
Service
Length
Specification
Note
Purification
Turnaround
sgRNA oligo
97–103 nt
1.5 nmol/3 nmol/5 nmol
First and last 3 bases with 2'-OMe and PS
OPC/HPLC
Inquire
crRNA
42 nt
5 nmol/2 OD
CRISPR-Cas system crRNA (Cas9, AsCas12a, LbaCas12a, LwCas13a, PspCas13b, RxCas13d available)
HPLC
Inquire
tracrRNA
64 nt
3 nmol/2 OD
CRISPR-Cas9 tracrRNA, can be duplexed with crRNA to form sgRNA
HPLC
Inquire
Service
Length
Specification
Modification
Purification
Application
gapmer-PS-ASO
16-20 nt
2 OD
5 PS at the 3′-end and 5′-end; central DNA gap; all PS backbone
HPLC
Generally used for cell experiments
gapmer-2-OMe-ASO
16-20 nt
2 OD
5 2'-OMe at the 3′-end and 5′-end; central DNA gap; all PS backbone
HPLC
Can be used for animal experiments
gapmer-2-MOE-ASO
16-20 nt
2 OD
5 MOE at the 3′-end and 5′-end; central DNA gap; all PS backbone
HPLC
Can be used for animal experiments
gapmer-2-LNA-ASO
16-20 nt
2 OD
3 LNA at the 3′-end and 5′-end; central DNA gap; all PS backbone
HPLC
Can be used for animal experiments
gapmer-cEt-ASO
16-20 nt
2 OD
3 cEt at the 3′-end and 5′-end; central DNA gap; all PS backbone
HPLC
Can be used for animal experiments
mixer-PS-ASO
16-20 nt
2 OD
PS-ASO, fully modified with PS backbone only
HPLC
Generally used for cell experiments
mixer-2-OMe-ASO
16-20 nt
2 OD
2'-OMe-ASO, fully modified with 2'-OMe + PS backbone
HPLC
Can be used for animal experiments
mixer-2-MOE-ASO
16-20 nt
2 OD
MOE-ASO, fully modified with MOE + PS backbone
HPLC
Can be used for animal experiments
mixer-LNA-ASO
16-20 nt
2 OD
LNA-ASO, fully modified with LNA + PS backbone
HPLC
Can be used for animal experiments
mixer-cEt-ASO
16-20 nt
2 OD
cEt-ASO, fully modified with cEt + PS backbone
HPLC
Can be used for animal experiments
Modification guidance: For therapeutic-program ASOs, MOE or cEt gapmer designs are recommended. For research-grade gene knockdown in cell culture, standard 2′-OMe or 2′-F gapmer ASOs are appropriate and more cost-effective. Contact our team to discuss the best design for your target.
Service
Specification
Purification
Note
Application
miRNA mimics
5 nmol/2 OD
HPLC
Double-stranded RNA, mimics endogenous mature miRNA, used for miRNA gain-of-function studies
in vitro transfection, requires transfection reagent
miRNA inhibitor
10 nmol/2 OD
HPLC
single-stranded, chemically modified, antisense oligonucleotide that binds to endogenous miRNA, used for miRNA loss-of-function studies
in vitro transfection, requires transfection reagent
mimics/inhibitor control（NC）
1 OD
HPLC
negative control with scrambled sequence, no homology to known miRNAs
in vitro negative control, use with mimics or inhibitor
miRNA agomir
2 OD
HPLC
chemically modified miRNA mimic with enhanced stability and cellular uptake, suitable for in vivo applications
in vivo (animal studies), in vitro (no transfection reagent required)
miRNA antagomir
2 OD
HPLC
chemically modified miRNA inhibitor with enhanced stability and cellular uptake, suitable for in vivo applications
in vivo (animal studies), in vitro (no transfection reagent required)
agomir/antagomir control（NC）
2 OD
HPLC
negative control for agomir/antagomir, chemically modified scrambled sequence
in vivo negative control, use with agomir or antagomir
Custom modified RNA (3–180 nt) with any combination from our 200+ modification catalog — including GalNAc conjugation, lipid attachment, and multi-position backbone chemistries — all with HPLC purification. Contact our team for a project consultation.
Order Workflow
From sequence to RNase-free delivery
01
Submit sequence & mods
Online or via email with full modification requirements.
02
Design review
Our team confirms modification placement, purification, and duplex annealing conditions.
03
Quality verification
All products verified by mass spectrometry before shipment.
04
RNase-free delivery
Lyophilized in RNase-free conditions with COA and optional MS chromatogram.
Key Advantages
Verified at every position
01
Full chemical modification support
All major therapeutic and research RNA modification chemistries available: 2′-OMe, 2′-F, PS, LNA, cEt, MOE, GalNAc, and more. Modifications verified by mass spectrometry at every position.
02
Mass spectrometry verification on every order
Every RNA oligonucleotide verified by mass spectrometry before shipment. COA includes molecular weight confirmation and modification integrity data.
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Ready-to-use, RNase-free delivery
All RNA products supplied lyophilized in RNase-free conditions with complete documentation. Fluorescently labeled RNA packaged in light-protective containers.
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Technical consultation
Our team provides guidance on modification selection, duplex design, delivery strategy, and experimental troubleshooting.
Applications
RNA research and therapeutic programs
Gene silencing (siRNA)
Chemically synthesized siRNA duplexes for RNAi-mediated gene knockdown in cell culture and in vivo studies.
Genome editing (sgRNA + Cas9)
Standard and modified sgRNA for CRISPR/Cas9 RNP delivery. No DNA carryover risk compared to IVT-derived guides.
Antisense regulation (ASO)
Gapmer ASOs for RNase H-dependent knockdown. Steric-blocking ASOs inhibit mRNA translation by occupying ribosome binding sites.
miRNA research
miRNA mimics and inhibitors for gain- and loss-of-function studies in cellular and in vivo models.
CRISPR screening libraries
Pooled sgRNA libraries with consistent synthesis and modification quality across hundreds to thousands of sequences.
RNA therapeutics research
Research-to-preclinical scale supply for ASO, siRNA, and modified RNA programs with full analytical documentation.
Questions about your RNA design?
Our team provides modification guidance, duplex design support, and custom quotes.
Eton Bioscience, Inc.
9215 Brown Deer Rd,
Suite B
San Diego, CA 92121
support@etonbio.com
North Carolina Branch
400 Park Offices Dr,
Suite 204
Research Triangle Park,
NC 27709
supportnc@etonbio.com
*Boston Branch
132 Charles Street,
Auburndale, MA 02466
Relocating – please send all
samples to our NC branch, pending re-opening of our MA Branch. supportma@etonbio.com
* Relocating – please send all samples to our NC branch, pending re-opening of our Boston Branch