Protein & Antibody
Protein & Antibody
Services
From recombinant protein expression to antibody discovery — Eton delivers research-ready proteins and antibodies for assay development, structural biology, therapeutic research, and diagnostics.
Key Capabilities
Expression systemsE. coli · Yeast · Insect · Mammalian, etc.
Codon optimizationIncluded on all projects
Antibody expressionAs fast as 1 week
Protein expressionAs fast as 2 weeks
Antibody puritySDS-PAGE & HPLC >95%
QC documentationCOA with every order
Overview
Research-ready proteins and antibodies, end to end
Eton provides research-ready proteins, peptides, and antibodies — from recombinant expression and custom peptide synthesis to full antibody discovery workflows. All projects are managed by our technical team, with complete QC documentation delivered with every order.
Services
Full protein and antibody portfolio
Protein
Recombinant Protein Expression
E. coli, B. subtilis, yeast, mammalian cells, baculovirus-insect, and cell-free system — tailored to your protein.
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Antibody
Antibody Services
Polyclonal & monoclonal antibodies, and recombinant antibodies in IgG, VHH, and Fab formats. End-to-end solutions with comprehensive documentation.
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Peptide
Custom Peptide Synthesis
Standard, modified, and conjugated peptides for antibody production, assay development, and structural studies.
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Discovery
Antibody Screening & Discovery
Single B-cell, phage display, and hybridoma platforms. IP ownership retained by the client.
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Key Advantages
End-to-end with complete documentation
01
Codon optimization included
Free codon optimization for all protein and antibody expression projects — no additional charge.
02
Comprehensive QC data
Each order comes with quality documentation, including purity and activity data, with additional analyses provided as needed.
03
Recombinant Antibodies in 2 Weeks
Recombinant antibodies (IgG, VHH-His, VHH-Fc, Fab) delivered in as little as 2 weeks, with sequence verification and QC data provided as appropriate.
04
Cell-Based Assay Reagents
Proteins and antibodies with purity and activity optimized for reliable assays.
Applications
Research to discovery
Target validation & functional studies
Recombinant proteins and antibodies for binding, activity, and pathway studies.
Antibody production for research & diagnostics
Custom monoclonal and polyclonal antibodies for ELISA, WB, IHC, and diagnostic assay development.
Structural biology
High-purity protein preparations for crystallography and cryo-EM studies.
Drug discovery
Protein and antibody reagents for hit identification and lead optimization programs.
Biosensor development
Functionalized proteins and antibodies for surface-based and solution-phase sensing applications.
Cell-based assay reagent production
Recombinant proteins and antibodies at purity and activity levels required for reliable cell-based assays.
Ready to start your protein or antibody project?
Our technical team handles project review, expression strategy, and custom quotes.
support@etonbio.com
Protein Services
Recombinant Protein
Expression
Recombinant protein expression across six expression systems, with full project support from sequence through to purified protein delivery.
Expression Systems
E. coliFast soluble proteins
B. subtilisSecretory expression
YeastGlycosylated proteins
Mammalian (CHO/HEK293)Human-like PTMs
Insect (baculovirus)Complex assemblies
Cell-free systemRapid, flexible expression
Overview
Six expression systems, one service team
Eton provides recombinant protein expression across six platforms — E. coli, B. subtilis, yeast, insect (baculovirus), mammalian (CHO/HEK293), and cell-free systems — plus custom peptide synthesis with HPLC purification. Our team manages project coordination and documentation, from sequence submission to purified, characterized protein delivery.
Expression Systems
Matched to your target protein
E. coli
Fast soluble proteins
The fastest and most cost-effective option for proteins that fold correctly in prokaryotic systems. Ideal for research-grade proteins where speed matters.
B. subtilis
Secretory expression
Gram-positive bacterial host with efficient secretion of recombinant proteins — reducing downstream purification complexity.
Yeast
Glycosylated proteins
Pichia pastoris or S. cerevisiae for proteins requiring eukaryotic post-translational modifications, particularly N-linked glycosylation.
Insect (Baculovirus)
Complex assemblies
Sf9 and High Five cells for multi-subunit complexes and large proteins requiring eukaryotic folding machinery and partial glycosylation.
Mammalian (CHO/HEK293)
Human-like PTMs
Full human-like post-translational modifications including complex glycosylation — preferred for therapeutic-context proteins and biologics.
Cell-Free System
Rapid, flexible expression
Cell-free transcription/translation for toxic proteins, non-standard amino acid incorporation, and rapid prototyping without cloning.
Order Workflow
From sequence to purified protein
01
Project review
Our team reviews your target sequence and discusses expression strategy.
02
Construct design
Codon optimization and vector cloning for selected expression system.
03
Expression
Protein production in selected host system.
04
Purification & QC
Affinity chromatography; purity, activity, and integrity testing. Delivery with COA.
Ready to discuss your protein expression project?
Our team selects the optimal expression system for your target protein.
support@etonbio.com
Antibody Services
Antibody Services —
from discovery to sequence-defined production
Polyclonal & monoclonal antibodies, and recombinant antibodies in IgG, VHH, and Fab formats — with complete QC documentation at every stage.
Recombinant Antibody Expression
TurnaroundAs fast as 2 weeks
SDS-PAGE purity>95%
HPLC purity>95%
Endotoxin<1 EU/mg
FormatsIgG · VHH-His · VHH-Fc · Fab
Key Advantages
High-purity antibodies with complete analytical documentation
01
Recombinant antibodies within 2 weeks
Full-length IgG, VHH-His, VHH-Fc, and Fab formats. Sequence-verified with SDS-PAGE and HPLC purity data provided as standard.
02
High-purity with complete analytical data
SDS-PAGE purity >95%, HPLC purity >95%, and endotoxin <1 EU/mg. Full QC report and COA provided with every order.
Order Workflow
Antibody development workflow
01
Antigen design
Peptide or protein antigen synthesis for immunization.
02
Immunization
Antibody induction in mice, rabbits, or other species.
03
Clone identification
Hybridoma generation or single B-cell screening.
04
Sequencing
Variable or full-length antibody gene sequencing.
05
Recombinant expression
Transient production in CHO or HEK293 cells.
06
Purification & QC
Affinity purification; SDS-PAGE, HPLC, and endotoxin testing. COA delivered.
Starting an antibody project?
Our technical team handles project review, format selection, and custom quotes.
support@etonbio.com
Peptide Synthesis
Custom Peptide Synthesis —
standard to fully modified peptides
Standard, modified, structured, conjugated, and library peptides — delivered with HPLC purity data and mass spectrometry confirmation.
At a Glance
Fast Turnaround2–3 weeks
Scalable Productionmg – kg
Flexible Purityup to >98%
QCMS + HPLC chromatogram
Service Details
Peptide services & specifications
| Service Type |
Features |
Specifications |
Turnaround |
| Standard Peptide Synthesis |
Suitable for peptides ≤30 amino acids |
1 mg–1 g; Crude to 75%–98% purity |
2–3 weeks |
| Modified Peptide Synthesis |
Lipidation, phosphorylation, cyclization, and labeling with fluorescent dyes or biotin |
Various modifications |
4–6 weeks |
| Structured Peptide Synthesis |
Provides linear, branched, asymmetric branched, and cyclic peptide structures |
Various peptide structures |
4–6 weeks |
| Peptide Conjugation |
Enables conjugation to KLH, BSA, OVA, and other carrier proteins |
Conjugation service for antigen peptides |
3–5 weeks |
| Peptide Library |
Suitable for single peptides, bulk peptides, or custom peptide libraries |
Bulk orders of peptide libraries |
3–5 weeks |
Applications
Common use cases
Antibody production
Antigen peptides for immunization, conjugated to carrier proteins (KLH, BSA, OVA) for enhanced immune response.
Assay development
High-purity peptides as standards, controls, or capture reagents in ELISA, competitive binding, and cell-based assays.
Structural biology
Defined peptide substrates, inhibitors, and probes for crystallography, NMR, and biochemical characterization.
Drug discovery
Modified peptides as tool compounds, protease substrates, or pharmacological probes for target validation.
Cell biology
Cell-penetrating peptides, receptor ligands, and fluorescently labeled peptides for live cell imaging and functional studies.
Screening libraries
Focused peptide libraries for epitope mapping, enzyme profiling, and high-throughput binding assays.
Need a custom peptide?
Contact our team to discuss your sequence, modification requirements, and purity specifications.
support@etonbio.com
Antibody Discovery
Antibody Screening & Discovery —
single B-cell, VHH, and hybridoma
High-throughput antibody discovery platforms for identifying antigen-specific monoclonal antibodies. All IP generated belongs to the client.
Platforms
Single B-cellUp to 10,000 cells/run
VHH (nanobody)High-throughput
HybridomaBatch processing
IP ownershipClient retains all rights
Service Details
Discovery platform specifications
| Platform | Throughput | Deliverables | Turnaround |
|---|
| Single B-cell screening | Up to 10,000 cells/run | VH/VL sequences + recombinant antibody | Per inquiry |
| VHH (nanobody) discovery | High-throughput | VHH sequence + expressed VHH | Per inquiry |
| Hybridoma | Batch processing | VH/VL variable domain sequence | Per inquiry |
IP ownership: All antibody sequences and associated intellectual property generated belong to the client — no licensing restrictions. End-to-end workflow from antigen design and immunization through screening, sequencing, and recombinant expression is available as a single project.
Order Workflow
From antigen to sequence-defined antibody
01
Antigen & immunization strategy
Our team prepares antigen and advises on immunization approach.
02
B-cell isolation or hybridoma generation
Single B-cell isolation or hybridoma fusion based on the selected platform.
03
Screening
Antigen-specific binding confirmed by ELISA or cell-based assay.
04
Sequencing
VH/VL or VHH variable domain sequencing.
05
Recombinant expression
Optional — convert sequence to recombinant IgG, Fab, or VHH.
06
Delivery
Sequence data, binding data, and purified recombinant antibody with COA.
Starting an antibody discovery project?
Contact our team to discuss your antigen, platform selection, and project scope.
support@etonbio.com