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Galactose Assay Kit

Galactose is a monosaccharide that can be found in milk, and it is involved in different biological processes in human body.Eton's Galactose Assay Kit provides a simple and reliable method for quantifying galactose in biological samples, such as serum,plasma,cell culture media etc. In the assay, Galactose is oxidized by enzyme reactions to yield color product, which can be measured at 570nm for colorimetric assay, and the color intensity is proportional to Galactose concentrations, therefore, the sample Galactose concentration can be accurately calculated based on the Galactose standards.

Sku #Product NameProduct SizePriceQTY
1200091002 Galactose Assay Kit 100 assays 100 assays $199.00 USD
120009100P Galactose Assay Kit- 100 Assays w/Reader Plate (1x) 100 assays $209.00 USD
 

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Sample Typle

Plasma,Serum,Cell culture supernatant, other body fluid

Components

Method

Colorimetric method at 570nm

Standard Curve

Sensitivity

20µM-1000µM

Reaction Time

30 minutes

Applications

For biological research:
Galactose measurement in biological samples
For drug/pharm research:
Drug influence on Galactose metabolism

Storage

-80°C

Notes

Material needed but not supplied

A plate reader capable of measuring absorbance at 570nm

Adjustable pipettes and a repeat pipettor

Distilled water(milliQ or HPLC-grade)

Clear Flat bottom 96-well plates if not included in the kit purchased

Shipping Icepacks

Reagent Preparation

Note: All reagents are frozen. We recommend you spin small vials before opening.


1. Galactose Assay Buffer(10x)

Note: Please don’t do dilution in the provided Galactose Assay Buffer bottle itself.


The bottle contains 10ml of 10x Galactose Assay Buffer. The assay buffer must be equilibrated to room temperature. Please make appropriate amount of 1xGalctose Assay Buffer for the assay, for example, by diluting 1ml of 10xGalactose Assay Buffer with 9 ml of distilled water. Prepare additional diluted assay buffer if needed. Store at -80˚C.

2. Galactose Standard (10x)

Note: Please don’t do dilution in the provided Galactose Standard vial itself.


The vial contains 100µl of 10mM Galactose Standard. The standard must be equilibrated to room temperature before use. Dilute 100µl of 10mM Galactose Standard with 900µl of diluted Galactose Assay Buffer to prepare a 1mM Galactose Standard. 1ml of diluted Galctose Standard is enough for making 3 standard curves if assayed in duplicate. Store at -80˚C.

3. Assay Probe

The vial contains 45μl of Assay Probe. The assay probe must be equilibrated to room temperature before use. It is sufficient for 100 wells.Protect from light. Store at -80˚C.

4. Galactose Enzyme Mix

The vial contains 35μl of Galctose Enzyme Mix. The enzyme mix must be thawed on ice before use. It is sufficient for 100 wells. Store at -80˚C.

Protocol

1.Sample Preparation

Serum,plasma, urine, other body fluids can be measured directly by a series of dilutions of the sample (1/2; 1/4; 1/8; …) to ensure the readings are within the standard curve range.Your samples can be diluted with dH2O or diluted Galactose Assay Buffer.

Add 50μl of samples to each well.
We recommend that samples be assayed in duplicate.

Note:
If prepared samples are not assayed the same day, store the samples at -80˚C. The samples will be stable for one month while stored at -80˚C. For frozen samples, dilutions of samples must be done right before assaying.

2.Standard Curve Prepation

Add 50μl, 40μl, 30μl, 20μl, 10μl, 5μl, 1μl, and 0μl of diluted Galactose Standard to each well, then adjust volume to 50μl/well with diluted Galactose Assay Buffer.
We recommend that Galactose Standards be assayed in duplicate. A standard curve has to be run in each assay.

3.Reaction Solution Preparation
50μl Reaction Mix is required for each well and 1ml of Reaction Solution is prepared with the followings:
986µlGalactose Assay Buffer(1x)

6µl GalactoseAssay Enzyme Mix
8µlAssay Probe


1ml of Reaction Solution is enough for ~20 wells.

Prepare appropriate amount of Reaction Solution for the number of assays to be performed.

Note:Please do not keep any leftover Reaction Solution as it has to be made fresh right beforeyou run assays. If old Reaction Solution is used, assays will not work.

4.Perform the assay

a. Mix the reaction solution well and add 50μl of the reaction solution to each well containing the Galactose standards and test samples.

b. Incubate the reaction for 30 minutes in a 37˚C incubator.

c. Measure absorbance at 570 nm in a microplate reader.

5. Calculation

a. Average the OD570 nm values of replicate wells of each Galactose Standard, test samples, and blank. Then, correct background by subtracting the value derived from the blank from all sample readings.

b. Make a standard curve by plotting OD570 nm values from each Galactose Standards as a function of Galactose concentration. This can be done with excel spreadsheet.

c. Calculate the value of Galactose in samples using the equation obtained from the linear regression of the standard curve.

Galactose (μM)= [ (Corrected absorbance)-(y-intercept)]/Slope

Material Safety Data Sheet

Date:Oct 26,2015

1. PRODUCT AND COMPANY IDENTIFICATION

1.1 Product identifier

Product name: Galactose Assay Kit

Catalog No: 1200091002,120009100P

1.2 Relevant identified uses of the substance or mixture and uses advised against

For research use only.

1.3 Details of the supplier of the safety data sheet

Company:Eton Bioscience Inc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4 Emergency telephone number

1-800-758-1630

2. HAZARDS IDENTIFICATION

2.1 Classification of the substance or mixture

Not a hazardous substance or mixture

2.2 GHS Label elements, including precautionary statements

Not a hazardous substance or mixture

2.3 Other hazards

None

3. COMPOSITION/INFORMATION ON INGREDIENTS

3.1 Substances

Galactose Assay Buffer

Galactose Assay Enzyme Mix

Components

CAS#

Components

CAS#

Potassium Phosphate Monobasic

7778-77-0

Potassium Phosphate Monobasic

7778-77-0

Potassium Phosphate Dibasic

7758-11-4

Potassium Phosphate Dibasic

7758-11-4

Water

7732-18-5

Galactose Oxidase

9028-79-9

Galactose Standard

Assay Probe

Components

CAS#

Components

CAS#

Sodium D-Galactose

59-23-4

DMSO

67-68-5

Potassium Phosphate Monobasic

7778-77-0

Potassium Phosphate Dibasic

7758-11-4

Water

7732-18-5












4. FIRST AID MEASURES

4.1 Description of first aid measures

Eye contact

Check for and remove contact lenses and flush with copious amounts of water; assure adequate flushing by separating the eyelids with fingers; call a physician

Skin Contact

Flush with copious amounts of water; remove contaminated clothing and shoes; call a physician

Inhalation

Remove to fresh air. If not breathing give artificial respiration ;call a physician

Ingestion

If swallowed, never give anything by mouth to an unconscious person. Rinse mouth with water ;call a physician

4.2 Most important symptoms and effects, both acute and delayed

No information available

4.3 Indication of any immediate medical attention and special treatment needed.

No information available

5. FIRE FIGHTING MEASURES

5.1 Extinguishing media

Suitable extinguishing media

Water spray, alcohol-resistant foam, dry chemical or carbon dioxide

5.2 Special hazards arising from the substance or mixture

No information available

5.3 Advice for firefighters

Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.

6. ACCIDENTAL RELEASE MEASURES

6.1 Personal precautions, protective equipment and emergency procedures

Use full personal protective equipment. Evacuate personnel to safe areas. Avoid breathing vapors,mist or gas.

6.2 Environmental precautions

Prevent further leakage or spillage. Prevent product from entering drain.

6.3 Methods and material for containment and cleaning up

Keep in suitable, closed containers for disposal

7. HANDLING AND STORAGE

7.1 Precautions for safe handling

Avoid skin/eye contact. Use protective equipment as needed. Wash contaminated clothing before reuse.

7.2 Conditions for safe storage,including any incompatibilities

Keep container tightly closed. Store at -80˚C.

7.3 Specific end use(s)

No data available.

8. EXPOSURE CONTROLS/PERSONAL INFORMATION

8.1 Control Parameters

This product contains no hazardous materials with occupational exposure limits.

8.2 Exposure controls

Engineering controls

Provide shower and eye wash station

Personal protective equipment

Eye Protection

Wear safety goggles

Skin Protection

Wear protective clothing

Hand Protection

Wear protective gloves

Respiratory protection

Wear NIOSH/MSHA approved respirators

9. PHYSICAL AND CHEMICAL PROPERTIES

9.1 Information on basic physical and chemical properties

Galactose Assay Buffer

Appearance

Liquid, colorless

pH

7.0

Melting point

No data available

Freezing point

No data available

Boiling point

No data available

Density

No data available

Water solubility

Soluble

Galactose Standard

Appearance

Liquid, colorless

pH

7.0

Melting point

No data available

Freezing point

No data available

Boiling point

No data available

Density

No data available

Water solubility

Soluble

Galactose Assay Enzyme Mix

Appearance

Liquid, yellow

pH

No data available

Melting point

No data available

Freezing point

No data available

Boiling point

No data available

Density

No data available

Water solubility

Soluble

Assay Probe

Appearance

Liquid, colorless

pH

No data available

Melting point

No data available

Freezing point

No data available

Boiling point

No data available

Density

No data available

Water solubility

Soluble

9.2 Other safety information

No data available.

10. STABILITY AND REACTIVITY

10.1Reactivity

No data available

10.2Chemical stability

Stable under recommended storage conditions.

10.3Possibility of hazardous reactions

No data available.

10.4Conditions to avoid

No data available

10.5Incompatible materials

Strong oxidizing agents

10.6Hazardous decomposition products

Hazardous decomposition products formed under fire conditions: carbon oxides

11. TOXICOLOGICAL INFORMATION

11.1Information on toxicological effects

Toxicological effects on this product have not been thoroughly studied.

12. ECOLOGICAL INFORMATION

12.1Toxicity

Avoid release into environment

12.2Persistence and degradability

No data available

12.3.Bioaccumulative potential

No data available

12.4Mobility in soil

No data available

12.5Results of PBT and vPvB assessment

No data available

12.6Other adverse effects

No data available

13. DISPOSAL INFORMATION

13.1Waste treatment methods

Dispose in accordance with local, state, and federal regulations.

14. TRANSPORT INFORMATION

DOT:

Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport
IATA:

Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport

ADR/RID

Proper shipping name: none
Non-Hazardous for transport: this substance is considered to be non-hazardous for transport

15. REGULATORY INFORMATION

EU Risk and Safety phrases:

R36/37/38: irritating to eyes/respiratory system/skin

16. OTHER INFORMATION

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information. Eton Bioscience Inc. shall not be held liable for any damages or other consequences resulting from handling or from contact with the above product.


Copyright © 2003-2018

All Rights Reserved

Eton Bioscience, Inc.
5820 Oberlin Drive, Suite 108
San Diego, CA 92121
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