D-Lactate Assay Kit
Glyoxalase II converts S-D-Lactoylglutathione and water into D-Lactate from the glyoxalase system pathway. The normal serum concentrations in mammals of D-Lactate are relatively low ranging in the nano to micromolar level. However during a bacterial infection in humans the serum concentration can rise to the millimolar range. High concentrations of D-lactate are indicative of ischemia, trauma or sepsis. The D-Lactate Assay Kit is based on the specifically oxidized D-lactate by D-Lactate dehydrogenase which exhibits an absorbance maximum at 490 nm. The intensity of the absorbance is proportional to the lactate concentration. Using a set of lactate standards, the assay can measure the concentration of lactate in samples (plasma, medium, or others) in a quantitative manner. The kit is stable until the expiration date of under proper storage and handling conditions. The product is for research only.