Gene Synthesis
Gene Synthesis
& Cloning
End-to-end gene synthesis, cloning, and plasmid construction — from sequence design to sequence-verified delivery. Supporting functional studies, protein expression, gene therapy vector development, and RNA therapeutics research.
At a Glance
Gene lengthUp to 200 kb
Standard turnaroundFrom 4 calendar days
Success rate≥99.5%
Vectors available200+
VerificationSanger + NGS
Codon optimizationIncluded as standard
Services
Complete gene-to-function solutions
Gene Synthesis
Standard & ProLong Gene Synthesis
Up to 200 kb, including GC-rich and repetitive sequences. From 4 calendar days for standard genes.
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Cloning
Gene Cloning & Mutagenesis
Seamless insertion into any vector, no restriction enzyme site limitations. 100% sequence accuracy.
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Plasmid Prep
Plasmid Preparation
Research-grade and endotoxin-free plasmid prep from 100 µg to 100 mg.
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IVT mRNA
IVT mRNA & LNP Encapsulation
Custom mRNA synthesis with full sequence optimization, capping, tailing, and optional LNP encapsulation.
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RNA Platforms
saRNA & Circular RNA
Self-amplifying RNA and circular RNA platforms for extended protein expression and enhanced nuclease stability.
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Key Advantages
Synthesis capabilities built for complex science
01
Genes up to 200 kb — including complex sequences
Long, GC-rich, and repetitive sequences that standard providers decline. Turnaround from 4 calendar days for standard genes.
02
Flexible vector options
200+ commercial vectors available, or insert into your specified vector. Codon optimization included as standard on all projects.
03
Guaranteed delivery — ≥99.5% success rate
Every gene sequence-verified by Sanger and NGS before shipment. Delivery committed, not estimated.
04
Difficult-sequence solutions
Toxic sequences, high GC content, repetitive elements — our team has mature solutions for improving synthesis success.
Applications
From research to preclinical programs
Protein expression research
Codon-optimized constructs for E. coli, yeast, insect, and mammalian expression systems.
Gene function studies
Overexpression, dominant-negative, and reporter constructs for functional characterization.
Gene therapy vector construction
ITR-flanked AAV payloads, lentiviral transfer plasmids, and regulatory element assemblies.
mRNA therapeutics
IVT mRNA, saRNA, and circRNA for research, vaccine, and therapeutic applications.
Pathway engineering
Multi-gene pathway assemblies for metabolic engineering and synthetic biology.
Synthetic biology
Custom gene circuits, regulatory elements, and large DNA assemblies up to 200 kb.
Ready to start your gene synthesis project?
Submit your sequence or request a custom quote from our technical team.
support@etonbio.com
Gene Synthesis
Gene Synthesis —
from simple sequences to 200 kb assemblies
Standard and ProLong gene synthesis — all sequence-verified before shipment.
Specifications
Standard turnaround5–22 calendar days
ProLong rangeUp to 200 kb
Success rate≥99.5%
Default vectorpUC57 or customer-specified
VerificationSanger + NGS
Service Details
Standard gene synthesis
| Gene length | Turnaround (calendar days) | Default vector | Deliverables |
|---|
| <1.5 kb | 5–8 | pUC57 or customer-specified | Lyophilized plasmid (1–4 µg); Sanger map; COA |
| 1.5–3 kb | 7–11 | Same | Same |
| 3–6 kb | 10–15 | Same | Same |
| 6–8 kb | 15–22 | Same | Same |
| >8 kb | Evaluation required | — | Contact our team |
ProLong Service
Large-scale & complex DNA synthesis
Designed for long, complex, or GC-rich sequences that exceed the limits of conventional gene synthesis. The ProLong service enables kilobase- to megabase-scale DNA delivery — including 50 kb industrial-scale fragments and assemblies up to 200 kb.
Order Workflow
From sequence to verified delivery
01
Submit your sequence
Online or via email. Specify vector preference and any special requirements.
02
Design review
Our team reviews for synthesis feasibility and suggests codon optimization if needed.
03
Sequence verification
Every gene verified by Sanger sequencing; NGS for complex assemblies.
04
Delivery
Lyophilized plasmid with sequencing data, COA, and stab culture.
Ready to submit your gene sequence?
Contact our team for synthesis feasibility review or a custom quote.
support@etonbio.com
Gene Cloning
Gene Cloning —
seamless insertion and precise mutagenesis
PCR cloning and site-directed mutagenesis without restriction enzyme site limitations. 100% sequence accuracy, verified by Sanger and NGS.
Specifications
MethodSeamless / restriction-free
Accuracy100% sequence verified
VerificationSanger + NGS
Mutagenesis windowAny 30-base window = 1 unit
Key Advantages
Cloning without constraints
01
No restriction enzyme site limitations
Insert any fragment into any vector, independent of available restriction sites in your sequence.
02
100% sequence accuracy
Every cloning project verified by Sanger and NGS before shipment. No ambiguous results delivered.
03
Any fragment into any vector
Compatible with all standard expression, reporter, and transfer vectors — or your custom vector.
04
Flexible mutagenesis pricing
Mutations within any 30-base window counted as a single unit — reducing project cost for clustered changes.
Questions about your cloning project?
Submit your insert and vector details for a feasibility review and quote.
support@etonbio.com
Plasmid Preparation
Plasmid Preparation —
research-grade to endotoxin-free
Research-grade and endotoxin-free plasmid preparation from 100 µg to 100 mg — for general molecular biology through cell-based and in vivo applications.
Specifications
Research GradePlasmid 1,000 ng/µL
Transfection GradeEndotoxin <0.1 / 0.01 / 0.005 EU/µg
Quantity range100 µg – 100 mg
Gene-to-PlasmidSaves 2 days vs. separate orders
Service Details
Two grades matched to your application
Research Grade
General molecular biology
Plasmid 1,000 ng/µL concentration. For transformation, cloning, PCR template, and transfection screening.
Transfection Grade
Cell-based assays & in vivo delivery
Endotoxin <0.1, 0.01, or 0.005 EU/µg — three levels to match your application stringency.
Gene-to-Plasmid: Combines gene synthesis and plasmid preparation in a single order, saving 2 days compared to ordering separately. Contact us with your sequence and required quantity.
Ready to order your plasmid prep?
Contact our team to discuss grade, quantity, and timeline.
support@etonbio.com
IVT mRNA
IVT mRNA —
custom mRNA synthesis and LNP encapsulation
Custom mRNA synthesis with full sequence optimization, in vitro transcription, capping, tailing, and optional LNP encapsulation — delivering research-ready materials for your program.
Service Includes
Sequence designUTR, CDS, PolyA optimization
CappingCo-transcriptional
Modified nucleotidesψ, N1ψ available
PurificationLiCl / bead / HPLC
LNP encapsulationOptional
Order Workflow
Six-step mRNA synthesis workflow
01
Sequence optimization
UTR, CDS, and PolyA tail design for stability and expression.
02
Template preparation
Gene synthesis, plasmid construction, and linearization.
03
RNA synthesis
In vitro transcription with co-transcriptional capping, tailing, and modified nucleotides (ψ, N1ψ).
04
Purification
LiCl, bead-based, or HPLC purification for high-purity product.
05
LNP encapsulation
Optional custom lipid nanoparticle formulation.
06
QC & delivery
RNA purity, capping efficiency, expression validation, and PolyA length analysis.
Starting an mRNA project?
Our team provides design consultation from sequence optimization through delivery format selection.
support@etonbio.com
RNA Platforms
Self-amplifying RNA (saRNA)
& Circular RNA (circRNA)
saRNA and circRNA platforms for extended protein expression and enhanced nuclease stability — available for research and preclinical applications.
Platform Comparison
saRNA basisAlphavirus replicon
circRNA stabilityNo free 5′/3′ termini
LNP encapsulationAvailable for both
Overview
Extended expression with advanced RNA platforms
Self-amplifying RNA (saRNA) incorporates a replicase element that amplifies intracellular RNA levels, enabling lower doses to achieve equivalent protein expression compared to standard mRNA. Circular RNA (circRNA) provides enhanced nuclease stability due to the absence of free 5′ and 3′ termini, extending protein expression without the need for modified nucleotides.
Service Details
Format specifications
| Format | Key feature | Delivery | Typical application |
|---|
| saRNA | Alphavirus replicon-based; lower dose requirement | LNP recommended | Vaccine research, protein expression |
| circRNA | No free termini; high nuclease resistance | LNP or naked | Extended expression studies, RNA therapeutics research |
Workflow for saRNA closely follows that of IVT mRNA. Key differences are the replicon design and the requirement for a longer RNA template. Contact our team to discuss your specific design requirements.
Exploring saRNA or circRNA?
Contact our team to discuss your sequence design and platform requirements.
support@etonbio.com